OLS unmixing of a Gating Set object
Usage
Luciernaga_Unmix(
x,
controlData,
sample.name,
removestrings,
Verbose,
addon,
subset = "root",
multiplier,
outpath,
PanelPath,
returntype = "fcs"
)Arguments
- x
A Gating Set object
- controlData
The matrix of single color controls generated from Luciernaga
- sample.name
The keyword containing the fcs file name
- removestrings
A list of values to remove from name
- Verbose
For troubleshooting name after removestrings
- addon
Additional addon to append to the new .fcs file name
- subset
A gating hierarchy level to sort cells at, expression values retrieved from these
- multiplier
A number to scale the OLS coefficients by
- outpath
The return folder for the .fcs files
- PanelPath
Location to a panel.csv containing correct order of fluorophores
- returntype
Whether to return "fcs" or "flowframe"