Iterates over single color variant .fcs files, passing the generated signature to unmix full-stain sample

Usage

Luciernaga_IterativeUnmixing(
  IterativePath,
  iterate_removestrings,
  removestrings,
  sample.name,
  subset = "root",
  PanelCuts,
  stats,
  Verbose,
  SignatureView,
  returntype = "data",
  FullStainedGS,
  controlData,
  multiplier,
  outpath,
  PanelPath
)

Arguments

IterativePath: File path to folder containing the variant single color .fcs files
iterate_removestrings: String values to remove from the variant .fcs files name
removestrings: Regular string values to remove to match single color name in controlData
sample.name: Keyword storage name
subset: What population to pass from GatingSet, default is root.
PanelCuts: A data.frame containing the cuts, see Luciernaga_Unmixing.
stats: Whether to use "median" or "mean" to derrive average signatures
Verbose: If TRUE returns steps
SignatureView: Whether to visualize the SCs
returntype: Default is set to "data" for iteration arguments
FullStainedGS: The GatingSet containing the full-stained samples you want to see effect of unmixing on
controlData: The Luciernaga_SingleColors output that will be used in combination with the iterated variant
multiplier: Default is 50000, amplifies the OLS output. Impacted by biexponential transformation
outpath: Desired storage location
PanelPath: Path to a reference panel for ordering of column markers in unmixed file

Value

Iterated versions of the full-stained sample

Examples

NULL
#> NULL