Visualize MFI of raw .fcs files to evaluate single color controls.

Usage

Luciernaga_Brightness(
  x,
  data,
  Downsample = TRUE,
  subsample = NULL,
  reference,
  clearance = 0.02,
  Scaled = TRUE
)

x: A passed Fluorophore name corresponding to those found in the data.
data: A data.frame containing the Luciernaga_FCSToReport raw MFI values.
Downsample: Whether to Downsample, default is set to TRUE to not overly affect the y-axis
subsample: If desired, a certain number of cells to take from each cluster. Default is NULL, selecting the number of cells found in the smallest cluster.
reference: A .csv path or data.frame containing Fluorophore and Detector info.
clearance: A buffer area multiplier to xmin and xmax when Scaled equals False
Scaled: Default is set to TRUE, returning logicle transformed MFI. FALSE returns raw MFI.

ggplot objects for each fluorophore containing the various clusters

NULL
#> NULL